In Glaxnimate timeline, if you open the Group 4 layer, then the Red Dot 1 layer, you’ll see that I used “Hold” type keyframes on the Opacity parameters.
“Hold” keyframes allows to change directly from one state to another. In that case from 0% to 100% opacity.
The first Hold keyframe is set at 100% opacity : The red dot will be visible at 100% opacity until the playhead reaches the next Hold keyframe that is set at 0% opacity. Then the red dot will stay at 0% opacity until the playhead reaches the next keyframe, set a 100% opacity… And so on…