Audio Quality decorated in each step of nesting

I don’t know why but the audio quality is being deteriorated after exporting the timeline to a clip even after trimming it and then exporting the video to a file. When I tried to nest a couple of *.mlt files into another timeline, in each step the quality is noticeably different even though I did not export anything, just played the timeline.

My own Preset name: MJPEG-HQ_50p

f=avi
acodec=pcm_s16le
ar=48000
ab=384k
vcodec=mjpeg
qscale=2
g=1
bf=0
progressive=1
top_field_first=0
deinterlace_method=linearblend
rescale=hyper
r=50
threads=0

If this is an issue, please resolve it, if not then I should point out my own faults, so please guide me.

I believe this was addressed in the audio changes for v16.12.